The Advantages of the Real Time PCR Method

Real Time PCR method

Among other gene expression profiling methods, RT PCR is fast becoming a staple in the field of plant biology. Over the last few years, technology for detecting PCR products has made a rapid leap forward. This method allows for enhanced genetic information, quantitative genotyping, allelic discrimination, and genetic variation. Several advantages of RT PCR make it a valuable tool in plant biology. Listed below are some of the benefits of using RT PCR. Click here –

Quantitative Analysis

The first step in the Real Time PCR method is to prepare samples. Then, DNA and RNA are extracted from the samples using a ready-to-use kit. Once the samples are prepared, the next step is to prepare the reaction. The reagents and sample should be carefully prepared in order to obtain accurate results. Afterwards, the samples should be placed in the machine. Alternatively, RT PCR can be used instead of agarose gel electrophoresis. Using the method, the concentration of a target gene can be determined based on the intensity of fluorescence.

The Real Time PCR method is widely used for quantitative analysis of mRNA levels. It uses a commercial thermocycler to amplify target nucleic acid sequences and simultaneously measure their concentration. It is a powerful tool in the field of biology, and it has made a huge impact on many fields. Despite its popularity, there is little consensus regarding the best Real Time PCR method. There are, however, several advantages.

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